9ÔÂ11ÈÕ£¬£¬£¬£¬£¬Science½ÒÏþ¿¹°©Ò©ÎïÇ°ÌåºÏ³É×îÐÂЧ¹û£¬£¬£¬£¬£¬À´×Ô˹̹¸£´óѧµÄÑо¿Ö°Ô±ÅжÏÁËÓÐÊýÖ²Îï¶ÜÒ¶¹í¾ÊÖйí¾Ê¶¾ËغϳÉ;¾¶ÖеÄÒªº¦Ã¸£¬£¬£¬£¬£¬²¢ÔÚÑ̲ÝÖоÙÐÐÒìÔ´±í´ï£¬£¬£¬£¬£¬Ê×´ÎÀֳɺϳɿ¹°©Ò©ÎïÒÀÍв´ÜÕµÄÇ°ÌåÎïÖÊÒÀÍв´ÜÕÌÇÜÕÅä»ù¡£¡£
ÿһ´ÎÊÖÒÕµÄË¢ÐÂ×Ü»á´øÀ´ÐµÄÎÄÃ÷£¬£¬£¬£¬£¬¾ÍÏñÕôÆû»úµÄ·¢Ã÷±ê¼Ç×ŵÚÒ»´Î¹¤Òµ¸ïÃüµÄ½µÉú¡£¡£ÏàÐÅ¿¹Å±¼²Ò©ÎïÇàÝïËáµÄÒìÔ´ºÏ³É¿°³ÆºÏ³ÉÉúÎïѧµÄÖ÷ҪˢУ¬£¬£¬£¬£¬ÔÚÒ»¶¨Ë®Æ½ÉÏÒ²ÏóÕ÷×Å×ÔÈ»²úÆ·ÒìÔ´ºÏ³É¸ïÃüµÄ½µÉú¡£¡£ÁÙ´²ËùÐè´ó×ÚÒ©ÎﶼȪԴÓÚÖ²Îï×ÔÈ»²úÆ·£¬£¬£¬£¬£¬È»¶øËüÃǵÄÉúÎïºÏ³É»ùÒòÈ´ÏÊΪÈËÖª£¬£¬£¬£¬£¬ÑÏÖصØ×è°ÁËÒìÔ´ºÏ³É¸¨ÖúÒ©ÎïÉú²ú¡£¡£ËäÈ»ÔÚÒìÔ´ËÞÖ÷ÖоÙÐÐ×ÔÈ»²úÆ·µÄºÏ³ÉÐèÒª½â¾öÖÖÖÖÄÑÌ⣬£¬£¬£¬£¬È»¶øÆÆÒëËùÐèµÄÉúÎï´ß»¯¼ÁÊÇÊ×µ±Æä³åµÄÎÊÌâ¡£¡£ÏÖÔÚÒÑÖªºÏ³É;¾¶²¢ÒѾʵÏÖ»òÔÚδÀ´ÓÐÍûʵÏÖÔÚÒìÔ´ËÞÖ÷Öй¤Òµ»¯Éú²úµÄ×ÔÈ»²úÆ·ÇüÖ¸¿ÉÊý£¬£¬£¬£¬£¬½öÓÐÇàÝïËá¡¢ÜлùÒìàßøÉúÎï¼îÀàÒÔ¼°µ¥ÝÆßÅßáÉúÎï¼îÀà¡£¡£È»¶ø×îÐÂÒ»ÆÚµÄScienceÔÙ´ÎΪºÏ³ÉÉúÎïѧ´øÀ´ÁËеÄÏ£Íû£¬£¬£¬£¬£¬À´×Ô˹̹¸£´óѧµÄÑо¿Ö°Ô±ÅжÏÁËÓÐÊýÖ²Îï¶ÜÒ¶¹í¾ÊÖйí¾Ê¶¾ËغϳÉ;¾¶µÄÒªº¦Ã¸À࣬£¬£¬£¬£¬²¢½«ÆäÔÚÑ̲ÝÖбí´ï£¬£¬£¬£¬£¬×îÖÕÊ״κϳÉÁËÒÀÍв´ÜÕµÄÇ°ÌåÎïÖÊ¡ª¡ªÒÀÍв´ÜÕÌÇÜÕÅä»ù£¬£¬£¬£¬£¬ÎªÒÀÍв´ÜյĻñÈ¡ÌṩÁËÒ»ÖÖ¸üÇå¾²¡¢ÓÐÓõķ½·¨¡£¡£×÷Ϊ¿¹°©Ò©ÎïµÄÇ°ÌåÎïÖÊ£¬£¬£¬£¬£¬ÒÀÍв´ÜÕÌÇÜÕÅà»ù½öÐèÒ»²½»¯Ñ§ºÏ³É±ã¿ÉµÃ¹âÁÙ´²ËùÓõÄÒ©Î£¬£¬£¬£¬Ò»¶¨¹ãÊܹØ×¢£¬£¬£¬£¬£¬ÄÇôËüµÄÒìÔ´ºÏ³ÉÊÇÔõÑùʵÏÖµÄÄØ£¿ £¿£¿£¿ £¿
1.ÒìÔ´ºÏ³ÉÖ²Îï×ÔÈ»²úÆ·µÄÐëÒªÐÔ
ÏÖÔÚÓÐÓõÄÒ©ÓÃÖ²Îï´úл²úÆ·¶¼ÈªÔ´ÓÚÖ²ÎïÌáÈ¡£¬£¬£¬£¬£¬ÈËÀà´ÓÖ²ÎïÖлñÈ¡Ò©Æ·µÄÀúÊ·ÓÉÀ´ÒѾ㬣¬£¬£¬£¬×ÝÈ»½ñÌìÒÀÈ»ÓÐԼĪһ°ëµÄÐÂÒ©À´×ÔÓÚÖ²Îï¡£¡£¿ÉÊÇÖ²Îï¹ÌÓÐÉú³¤»ºÂýµÄÈõµã£¬£¬£¬£¬£¬ÓÐЩÓÖÊôÓÚ´¦ÓÚ±ôΣ״̬µÄÓÐÊýÖ²Î£¬£¬£¬£¬¸øÖ²ÎïÔ´Ò©ÎïµÄÓ¦ÓôøÀ´ÄÑÌâ¡£¡£Ï²ÂíÀÑŶÜÒ¶¹í¾Ê¾ÍÊÇÒ»¸öºÜºÃµÄÀý×Ó£¬£¬£¬£¬£¬Æä×ÔÉíÄܹ»ºÏ³É¿¹°©Ò©ÎïÒÀÍв´ÜÕµÄÇ°Ìå¹í¾Ê¶¾ËØ£¬£¬£¬£¬£¬ÃÀ¹ú×Ô1983ÄêÆ𣬣¬£¬£¬£¬±ãÆձ齫ÒÀÍв´ÜÕÓ¦ÓÃÓڷΰ©¡¢ÁÜ°Í°©µÈ°©Ö¢µÄÖÎÁÆ¡£¡£ÏÖ½ñ¹í¾Ê¶¾ËØÖ÷ÒªÊÇ´ÓÃÀ¹ú¶ÜÒ¶¹í¾Ê»ñµÃ£¬£¬£¬£¬£¬¿ÉÊǽϳ¤µÄÉú³¤ÖÜÆںͽϵ͵IJúÁ¿ÏÞÖÆÁ˸ÃÒ©ÎïµÄÆÕ±éʹÓᣡ£ÈôÊÇʵÏÖÒìÔ´ºÏ³É£¬£¬£¬£¬£¬¹æÄ£»£»£»£» £»£»¯×÷ÓýÒ×ÓÚ²Ù×÷µÄËÞÖ÷£¬£¬£¬£¬£¬ÈçÑ̲ݡ¢½ÍĸÒÔ¼°´ó³¦¸Ë¾úµÈϸ°û£¬£¬£¬£¬£¬ÏàÐŲ»µ«ÄÜÍƶ¯ºÏ³ÉÉúÎïѧµÄ¸ïÃüÀ˳±£¬£¬£¬£¬£¬¸ü½«³ÉΪÔ츣ÈËÀàÒ½ÁÆ¿µ½¡µÄÐÛΰ¹¤³Ì¡£¡£
2.ÒÀÍв´ÜÕÓëÆÆÒëÊÂÇéµÄÒâÒåÊÂʵÊÇʲô£¿ £¿£¿£¿ £¿
ÒÀÍв´ÜÕ×÷ÊÇÓÉ»îÐÔ×ÔÈ»²úÆ·¹í¾Ê¶¾Ëؾ°ëºÏ³É»ñµÃµÄÑÜÉúÎ£¬£¬£¬£¬Í¨¹ýÒÖÖÆÍØÆËÒ칹øIIÀ´ÒýÆðDNAË«Á´¶ÏÁÑ£¬£¬£¬£¬£¬×èÖ¹°©Ï¸°ûµÄ¿ìËÙÆÆË飬£¬£¬£¬£¬Òò´Ë±»ÓÃÓÚ»¯ÁƶàÖÖ¶ñÐÔÖ×Áö¶øÆÕ±éÓ¦ÓÃÓÚÁÙ´²¡£¡£ËäÈ»ÒÀÍв´ÜÕ±»ÌìÏÂÎÀÉú×éÖ¯ÁÐΪ±ØÐèµÄÒªº¦Ò©Æ·£¬£¬£¬£¬£¬µ«ÆäÉú²úÀú³ÌÕÕ¾ÉÒÀÀµÓÚ´Ó¶ÜÒ¶¹í¾ÊÖÐÌáÈ¡Ç°ÌåÎïÖʹí¾ÊËØ£¬£¬£¬£¬£¬ÔÙ¾ÙÐл¯Ñ§·¨°ëºÏ³É¡£¡£
Òò´ËÆÈÇÐÐèÒªÍØ¿íÒÀÍв´ÜÕÇ°ÌåµÄȪԴ£¬£¬£¬£¬£¬Ê×ÏÈËäÈ»ÐèÒª½â¾ö×î´óµÄÕÏ°£¬£¬£¬£¬£¬ÆÆÒë¶ÜÒ¶¹í¾ÊÖеĹí¾ÊËغϳɻùÒò¡£¡£ÏàÐÅÕâÊÇ×÷ÕߵijõÖ¾£¬£¬£¬£¬£¬È»¶ø×îºóµÄЧ¹ûÖУ¬£¬£¬£¬£¬»ñµÃÆÆÒëµÄ;¾¶ÖÕ²úÆ·²¢²»Êǹí¾ÊËØ£¬£¬£¬£¬£¬¶øÊǹí¾ÊËØ;¾¶ÖеÄÁíÒ»¸öÎïÖÊ£¬£¬£¬£¬£¬ÒÀÍв´ÜÕÌÇÜÕÅä»ù£¨4¡ä-desmethylepipodophyllotoxin£©¡£¡£Õâ¸öÎïÖʱȹí¾ÊËظüÊʺϾÙÐÐÒÀÍв´Üյĺϳɣ¬£¬£¬£¬£¬Ò²¾ÍÊÇÃâÈ¥ÁËÔÚ»¯Ñ§ºÏ³ÉÖжԹí¾ÊËؾÙÐÐÒì¹¹»¯ºÍÈ¥¼×»ù»¯µÄ°ì·¨£¬£¬£¬£¬£¬Ö±½ÓºÏ³ÉÒÀÍв´ÜÕ¡£¡£
3.ÒÀÍв´ÜÕ(etoposide)Ç°ÌåÎïÖʺϳÉ;¾¶µÄÆÆÒëÀú³Ì
ÔÚ¶ÜÒ¶¹í¾ÊÖоÙÐлùÒò̽¿óÊǺÜÊÇÄÑÌâµÄ£¬£¬£¬£¬£¬ÓÉÓÚÕâÖÖÓÐÊýÖ²ÎïÉú³¤»ºÂý¡¢»ùÒò×éÖØ´ó£¨Ô¼16Gb£©,δ²âÐò£¬£¬£¬£¬£¬²¢ÇÒ¹¹½¨Í»±äÌåµÄÒªÁìÊ®·Ö·±Ëö¡£¡£ÎÄÕÂÖ÷Òª½ÓÄÉRNA-seqÆÊÎö¡¢Õ¹ÍûÂÑ°×¹²±í´ïÒÔ¼°ÖÊÆ×ÆÊÎöµÄÒªÁìÀ´ÆÆÒëÒÀÍв´ÜÕÌÇÜÕÅä»ùºÏ³ÉµÄÏà¹Øø¡£¡£ÓÉÓÚÑ̲ÝÄÜͨ³£ÄÜ׼ȷ±í´ï²î±ðµÄÂÑ°×£¬£¬£¬£¬£¬Í¬Ê±¾ßÓпìËÙÉú³¤¡¢Ò×ÓÚ²Ù×÷µÄÓŵ㣬£¬£¬£¬£¬±»Ñ¡Îª±¸Ñ¡»ùÒòÒìÔ´±í´ïµÄËÞÖ÷¡£¡£¾ÓÉÒÑÖªÂÑ°×DIR¡¢PLR¡¢SDHÓëCYP718A23ÔÚÑ̲ÝÖй²±í´ïÒÔ¼°ÖÊÆ×ÆÊÎöÖÐÐIJúÆ·ºó£¬£¬£¬£¬£¬SattelyÍŶӷ¢Ã÷ΪÑ̲Ýϸ°ûÌṩµ×ÎïÂį́Ê÷Ö¬´¼£¨matairesinol£©ºó¸üÄÜÓÐÓûýÀÛÖÐÐIJúÆ·pluviatolide£¨Í¼1£©£¬£¬£¬£¬£¬Òò´ËÒԴ˵×ÎïΪÆðµã£¬£¬£¬£¬£¬Æô¶¯Á˹í¾ÊËØ£¨podophyllotoxin£©µÄÆÆÒëÂó̡£¡£
¶ÜÒ¶¹í¾ÊºÏ³É¹í¾Ê¶¾ËØÓÃÓÚ±£»£»£»£» £»£»¤×ÔÉíÃâÊܲ¡³æº¦ÇÖȾ£¬£¬£¬£¬£¬Ö»Óе±Ò¶Æ¬Êܵ½Î£ÏÕʱ£¬£¬£¬£¬£¬¹í¾Ê¶¾ËزŻãºÏ³É¡£¡£SattelyÍŶӽø¶øÍƲâÓëΣÏÕÓ¦¼¤·´Ó¦Ïà¹ØµÄÂѰ׿ÉÄܼÓÈëµ½¹í¾Ê¶¾ËصĺϳÉ,ͨ¹ý½ÏÁ¿Ò¶Æ¬ÊÜÉËÇ°ºóRNA-seqÊý¾Ý£¬£¬£¬£¬£¬¼ì²âÂѰ׵ıí´ïˮƽ£¬£¬£¬£¬£¬²¢Õ¹ÍûÐèÒªÌî²¹¿Õȱ·´Ó¦µÄøÀà:O-¼×»ùתÒÆø£¨OMTs£©¡¢Ï¸°ûÉ«ËØP450(CYPs)¡¢2-ÑõÎì¶þËáÒÀÀµµÄË«¼ÓÑõø£¨2-ODDs£©ÒÔ¼°¶àÔª·ÓÑõ»¯Ã¸£¨PPOs£©(ͼ2)¡£¡£ÄÇÔõÑùÑ°ÕÒµ½ÈÏÕæÕâÁù¸öøºÏ³ÉµÄ»ùÒòÄØ£¿ £¿£¿£¿ £¿»ùÓÚ±í´ïÆ×µÄÅÌËãÆÊÎöÌìÉúÁË7¸öºòÑ¡»ùÒò¡£¡£Ñо¿Õß½øÒ»²½Í¨¹ýø¼Ò×å·ÖÀàɸѡ¶ÜÒ¶¹í¾ÊÖÐ34,384¸ö»ùÒòºó£¬£¬£¬£¬£¬¶ÔRNA-seqÊý¾Ý¾ÙÐÐÁË·Ö¼¶Èº¾Û£¬£¬£¬£¬£¬ÆÊÎöÁË336¸ö»ùÒò£¬£¬£¬£¬£¬Õ¹ÏÖÁËÒ»¸ö½ø»¯Ö¦ÉϵÄ91¸ö»ùÒò£¬£¬£¬£¬£¬½øÒ»²½Í¨¹ý±í´ïË®ÖÐÆÊÎö½«Õâ¸ö½ø»¯Ö¦Å¨ËõΪ22¸ö»ùÒò£¬£¬£¬£¬£¬ÆäÖаüÀ¨ÁË6¸öÒѾÍƲâµÄºòÑ¡»ùÒò¡£¡£Òò´ËÑо¿ÕßÒÔÂį́Ê÷Ö¬´¼Îª³ö·¢µ×ÎïÖð²½ÔÚÑ̲ÝÖй²±í´ï6¸öºòÑ¡»ùÒò£¬£¬£¬£¬£¬ÆÆÒëÁËÒÀÍв´ÜÕÇ°ÌåÒÀÍв´ÜÕÌÇÜÕ£¨4¡ä-desmethylepipodophyllotoxin£©Åä»ùµÄ;¾¶£¬£¬£¬£¬£¬²¢ÔÚÑ̲ÝÖй²±í´ïÕûÌõ;¾¶µÄ10¸ö»ùÒò£¬£¬£¬£¬£¬»ñµÃÁËÒÀÍв´ÜÕÌÇÜÕÅä»ùµÄÒìÔ´ºÏ³É,ÏÖÔÚ²úÂÊΪ10.3ng/mgÑ̲ݸÉÖØ¡£¡£
ËäÈ»ÒìÔ´ºÏ³É×îÖÕÄ¿µÄÕÕ¾ÉÒª×ßÏò½ÍĸËÞÖ÷µÄ£¬£¬£¬£¬£¬ÏÖÔÚÊÇ·ñÒÑÓÐÑо¿ÍŶÓÕýÔÚÆð¾¢ÖÐÄØ£¿ £¿£¿£¿ £¿ÎÒÃǽ«Ò»Á¬¹Ø×¢¡£¡£
ÕªÒë×Ô£ºhttp://www.sciencemag.org/content/349/6253/1224?related-urls=yes&legid=sci;349/6253/1224